Reporter

Part:BBa_J58101:Design

Designed by: Valencia iGEM 2006   Group: iGEM06_Valencia   (2006-10-26)


Promoter activated synergistically by cI and CRP with the reporter RFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 11
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 11
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 11
    Illegal AgeI site found at 687
    Illegal AgeI site found at 799
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Notice that in the promoter sequence the XbaI restricction site is present. Therefore, we have to digest this part upstream only with EcoRI.


Source

To make this composite part we assemby two parts from the Registry (a promoter and a generator).

References